| Fermate | Arrivo | Partenza | Binario |
|---|---|---|---|
| Pisa Centrale | 19:45 | 4 | |
| Livorno Centrale | 20:00 | 20:03 | 4 |
| Rosignano | 20:19 | 20:20 | 1 |
| Cecina | 20:28 | 20:29 | 2 |
| San Vincenzo | 20:42 | 20:43 | 2 |
| Campiglia Marittima | 20:50 | 20:51 | 1 |
| Follonica | 21:01 | 21:02 | 2 |
| Montepescali | 21:19 | 21:20 | 2 |
| Grosseto | 21:28 | 21:30 | 1 |
| Albinia | 21:45 | 21:46 | 1 |
| Orbetello | 21:52 | 21:53 | 3 |
| Capalbio | 22:02 | 22:03 | 1 |
| MONTALTO DI CASTRO | 22:17 | 22:18 | 1 |
| Tarquinia | 22:28 | 22:29 | 1 |
| Civitavecchia | 22:42 | 22:44 | 3 |
| Santa Marinella | 22:50 | 22:51 | 3 |
| Marina Di Cerveteri | 23:00 | 23:01 | 1 |
| Ladispoli-Cerveteri | 23:04 | 23:05 | 3 |
| Roma San Pietro | 23:24 | 23:25 | 4 |
| Roma Trastevere | 23:29 | 23:30 | 1 I |
| Roma Ostiense | 23:36 | 23:37 | 1 |
| Roma Tuscolana | 23:42 | 23:43 | 1 |
| Roma Termini | 23:48 | - |
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