| Fermate | Arrivo | Partenza | Binario |
|---|---|---|---|
| Chiusi-Chianciano Terme | 05:00 | 7 | |
| Castiglion Del Lago | 05:11 | 05:12 | 2 |
| Terontola-Cortona | 05:18 | 05:19 | 3 |
| Camucia-Cortona | 05:24 | 05:25 | 2 |
| Castiglione Fiorentino | 05:31 | 05:32 | 3 |
| Arezzo | 05:42 | 05:43 | 4 |
| Montevarchi-Terranuova | 06:04 | 06:05 | 2 |
| San Giovanni Valdarno | 06:09 | 06:10 | 2 |
| Figline Valdarno | 06:15 | 06:16 | 2 |
| Incisa | 06:20 | 06:21 | 2 |
| Rignano sull'Arno | 06:27 | 06:28 | 1 |
| Pontassieve | 06:34 | 06:35 | 4 |
| Firenze Campo Marte | 06:48 | 06:49 | 3 |
| Firenze Santa Maria Novella | 06:56 | - |
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